What is the NOAEL for Methylene blue?
Methylene blue has 34 NOAEL studies in the database. The lowest reported value is 0.25 mg/kg bw/day via injection in Dog. Source: ToxValDB_ECHA_IUCLID.
NOAEL Data
Cosmetic Ingredient
Methylene blue
Also known as: D and C Blue No. 1, D And C Blue Number 1, C.I. Basic Blue 9, M-B Tabs, Aizen Methylene Blue BH (+15 more)
CAS 61-73-4
Methylene blue (CAS 61-73-4) is a chemical substance. Key regulatory status: 8 regulatory/inventory lists, cosmetic ingredient cross-reference; source data from EPA ToxValDB, EPA CPDat.
View cosmetic safety profile for Basic Blue 9 (Methylene Blue) →
SOURCE
noael studies public
NOAEL studies
34
SOURCE
chemical inventory jurisdictions
Regulatory lists
8
SOURCE
eu clp annex vi
GHS signal
Not classified
Chemical Identity
CAS, identifiers, formula, and alternate names for the matched substance record.
SOURCE
DSSTox identifiers
26 fields
Name
Methylene blue
CAS Number
61-73-4
DTXSID
DTXSID0023296
Molecular Formula
C16H18N3S.ClH
InChI Key
CXKWCBBOMKCUKX-UHFFFAOYSA-M
Monoisotopic Mass
320.098821
Synonyms
D and C Blue No. 1D And C Blue Number 1C.I. Basic Blue 9M-B TabsAizen Methylene Blue BHAizen Methylene Blue FZCalcozine Blue ZFCaswell No. 567Ceruleum methylenumCI Basic Blue 9EINECS 200-515-2EPA Pesticide Chemical Code 039505
Functional Uses
Industrial and product-use categories associated with this substance.
SOURCE
EPA CPDat
2 records
Dye
Regulatory Lists
Inventory, screening, and regulatory list matches from public chemical databases.
SOURCE
EPA CPDat
8 records
| List | Keyword | Source |
|---|---|---|
| inert_ingredient; non_food_use; Pesticides | EPA | CPCat |
| CEDI | FDA | United States Food and Drug Administration (FDA) |
| Indirect additives food contact (10/2018) | FDA | United States Food and Drug Administration (FDA) |
| Canada; pharmaceutical | DrugBank | DrugBank |
| Canada; pharmaceutical | DrugBank | DrugBank |
| pharmaceutical | US International Trade Commission | CPCat |
| active_ingredient; Europe; Pesticides | Commission of the European Communities | CPCat |
| banned; Europe; Personal care | European Commission | CosIng |
NOAEL Studies
Toxicology endpoints rendered from public NOAEL study rows.
SOURCE
NOAEL studies
Showing 34 of 34 studies
| Value | Unit | Endpoint | Route | Species | Source |
|---|---|---|---|---|---|
| 18.5295744813443 | uM | AC50 | - | - | NTP_ICE_endocrine |
| 48.3519738192217 | uM | AC50 | - | - | NTP_ICE_endocrine |
| 6.790072082 | uM | ACC | - | - | NTP_ICE_endocrine |
| 17.5220106521944 | uM | ACC | - | - | NTP_ICE_endocrine |
| 13.9 | uL/min/10^6 cells | Clint | - | Human | NTP_ICE_adme_parameters |
| 8NAP7826UB | UNII | FDA UNII substance identifier | - | - | openFDA substances |
| 8NAP7826UB | UNII | FDA UNII substance identifier | - | - | openFDA substances |
| 8NAP7826UB | UNII | FDA UNII substance identifier | - | - | openFDA substances |
| 8NAP7826UB | UNII | FDA UNII substance identifier | - | - | openFDA substances |
| 0.38 | fraction | Fu | - | Human | NTP_ICE_adme_parameters |
| =1180 | mg/kg bw | LD50 | oral | Rat | NTP_ICE_acute_oral |
| >=0.25 | mg/kg bw/day | LEL | injection | Dog | ToxValDB_ECHA_IUCLID |
| >=0.5 | mg/kg bw/day | LEL | injection | Dog | ToxValDB_ECHA_IUCLID |
| =1 | mg/kg bw/day | LEL | injection | Dog | ToxValDB_ECHA_IUCLID |
| >=25 | mg/kg bw/day | LEL | oral | Rat | ToxValDB_ECHA_IUCLID |
| >=50 | mg/kg bw/day | LEL | oral | Rat | ToxValDB_ECHA_IUCLID |
| >=100 | mg/kg bw/day | LEL | oral | Rat | ToxValDB_ECHA_IUCLID |
| =200 | mg/kg bw/day | LEL | oral | Rat | ToxValDB_ECHA_IUCLID |
| - | unitless | Model Score | - | - | NTP_ICE_endocrine |
| 0.00154 | unitless | Model Score | - | - | NTP_ICE_endocrine |
| 0.0591 | unitless | Model Score | - | - | NTP_ICE_endocrine |
| 0.237 | unitless | Model Score | - | - | NTP_ICE_endocrine |
| <=1 | mg/kg bw/day | NOEL | intravenous | dog (Beagle; male/female) | FDA_Toxicity_9dca54be-d7e9-4fbd-aac8-155d2777da95_dossier.i6z |
| <=1 | mg/kg bw/day | NOEL | intravenous | dog (Beagle; male/female) | FDA_Toxicity_9dca54be-d7e9-4fbd-aac8-155d2777da95_dossier.i6z |
| <=1 | mg/kg bw/day | NOEL | intravenous | dog (Beagle; male/female) | FDA_Toxicity_9dca54be-d7e9-4fbd-aac8-155d2777da95_dossier.i6z |
| <=1 | mg/kg bw/day | NOEL | intravenous | dog (Beagle; male/female) | FDA_Toxicity_9dca54be-d7e9-4fbd-aac8-155d2777da95_dossier.i6z |
| <=50 | mg/kg bw/day | NOEL | 2207 | mouse (B6C3F1; male/female) | FDA_Toxicity_9dca54be-d7e9-4fbd-aac8-155d2777da95_dossier.i6z |
| <=50 | mg/kg bw/day | NOEL | 2207 | mouse (B6C3F1; male/female) | FDA_Toxicity_9dca54be-d7e9-4fbd-aac8-155d2777da95_dossier.i6z |
| <=50 | mg/kg bw/day | NOEL | 2207 | mouse (B6C3F1; male/female) | FDA_Toxicity_9dca54be-d7e9-4fbd-aac8-155d2777da95_dossier.i6z |
| <=50 | mg/kg bw/day | NOEL | 2207 | mouse (B6C3F1; male/female) | FDA_Toxicity_9dca54be-d7e9-4fbd-aac8-155d2777da95_dossier.i6z |
| <=350 | mg/kg bw/day | NOEL | oral: gavage | rat (Sprague-Dawley; male/female) | FDA_Toxicity_9dca54be-d7e9-4fbd-aac8-155d2777da95_dossier.i6z |
| <=350 | mg/kg bw/day | NOEL | oral: gavage | rat (Sprague-Dawley; male/female) | FDA_Toxicity_9dca54be-d7e9-4fbd-aac8-155d2777da95_dossier.i6z |
| <=350 | mg/kg bw/day | NOEL | oral: gavage | rat (Sprague-Dawley; male/female) | FDA_Toxicity_9dca54be-d7e9-4fbd-aac8-155d2777da95_dossier.i6z |
| <=350 | mg/kg bw/day | NOEL | oral: gavage | rat (Sprague-Dawley; male/female) | FDA_Toxicity_9dca54be-d7e9-4fbd-aac8-155d2777da95_dossier.i6z |
Showing 34 of 34 studies
Cosmetic Safety Profile
Same-CAS cosmetic ingredient record for cross-vertical context.
SOURCE
CosIng / Ingredients DB
EU Status
permitted
Max
-
Category
Colorant
Pharmaceutical Data
Same-CAS pharmaceutical records from drug and bioactivity sources.
SOURCE
EMBL-EBI ChEMBL
METHYLENE BLUE ANHYDROUS
ChEMBL Phase
4
Adverse Events
23
Bioactivity
17
Frequently Asked Questions
What regulatory lists include Methylene blue?
Methylene blue appears on 7 regulatory/inventory lists including inert_ingredient; non_food_use; Pesticides, CEDI, Indirect additives food contact (10/2018), and 4 more. Source: EPA CPDat.
Is Methylene blue used in cosmetics?
Yes, Methylene blue is also indexed as a cosmetic ingredient under the name Basic Blue 9 (Methylene Blue). View the full cosmetic safety profile on the ingredient page for detailed safety data, SCCS opinions, and regulatory status.
Where does the safety data for Methylene blue come from?
Safety data is sourced from EPA ToxValDB, EPA CPDat, EPA DSSTox, CosIng / Ingredients DB, ChEMBL / DailyMed. All data traces to primary regulatory sources and is updated from official government databases.
Is Methylene blue used outside industrial chemicals?
Methylene blue also appears in cosmetics, pharmaceutical databases.