NOAEL Data IARC Group 3 REACH Registered Cosmetic Ingredient

HC Blue 2

Also known as: HC Blue No. 2, BRN 2148848, EINECS 251-410-3, 2,2'-((4-((2-Hydroxyethyl)amino)-3-nitrophenyl)imino)bis(ethanol), NCI-C54897 (+5 more)

CAS 33229-34-4

HC Blue 2 (CAS 33229-34-4) is a chemical substance; key hazard signal: IARC Group 3. Key regulatory status: REACH registered, 5 regulatory/inventory lists, cosmetic ingredient cross-reference; source data from EPA ToxValDB, EPA CPDat, AICIS, SCCS, IARC, ECHA REACH.

View cosmetic safety profile for HC Blue No. 2 →

SOURCE NOAEL studies

NOAEL studies

SOURCE Chemical inventory jurisdictions

Regulatory lists

SOURCE EU CLP Annex VI

GHS signal

Not classified

Chemical Identity

CAS, identifiers, formula, and alternate names for the matched substance record.

SOURCE DSSTox identifiers 15 fields

Name

HC Blue 2

CAS Number

33229-34-4

Molecular Formula

C12H19N3O5

InChI Key

MIWUTEVJIISHCP-UHFFFAOYSA-N

Monoisotopic Mass

285.132471

Synonyms

HC Blue No. 2BRN 2148848EINECS 251-410-32,2'-((4-((2-Hydroxyethyl)amino)-3-nitrophenyl)imino)bis(ethanol)NCI-C548973-Nitro-N(sup 1),N(sup 1),N(sup 4)-tris(2-hydroxyethyl)-p-Phenylenediamine, 3-nitro-N(sup 1),N(sup 1),N(sup 4)-tris(2-hydroxyethyl)-N(sup 1),N(sup 4),N(sup 4)-Tris(2-hydroxyethyl)-2-nitro-para-phenylenediamineUNII-6C0EL1931V2,2'-[[4-[(2-Hydroxyethyl)amino]-3-nitrophenyl]imino]bis-ethanol

IARC Carcinogen Classification

Carcinogenic hazard classification from IARC monograph evaluations.

SOURCE IARC Monographs on the Identification of Carcinogenic Hazards to Humans 1 records

Group	Meaning	Evaluated	Volume
Group 3	Not classifiable	1992	57

Functional Uses

Industrial and product-use categories associated with this substance.

SOURCE EPA CPDat 4 records

Dye

Regulatory Lists

Inventory, screening, and regulatory list matches from public chemical databases.

SOURCE EPA CPDat 5 records

List	Keyword	Source
Europe; Personal care; restricted	European Commission	CosIng
Europe; Personal care - hair coloring	Danish Environmental Protection Agency	Danish EPA
children; Europe; Personal care	Danish Environmental Protection Agency	Danish EPA
children; Europe; Personal care	Danish Environmental Protection Agency	Danish EPA
children; Europe; Personal care	Danish Environmental Protection Agency	Danish EPA

NOAEL Studies

Toxicology endpoints rendered from public NOAEL study rows.

SOURCE NOAEL studies Showing 24 of 24 studies

Value	Unit	Endpoint	Route	Species	Source
100	mg/kg bw/day	-	-	human	Regulatory source
300	mg/kg/day	-	-	-	Regulatory source
300	mg/kg bw/day	-	-	human	Regulatory source
1000	mg/kg/day	-	oral	rat	Regulatory source
6C0EL1931V	UNII	FDA UNII substance identifier	-	-	openFDA substances
3	IARC group	IARC carcinogenicity classification	-	-	IARC Monographs
3	unitless	IARC group	-	-	NTP ICE cancer
=0.0004	mg/kg	NOAEL	oral	rat	SCCS Opinion
=100	mg/kg bw/day	NOAEL	-	rat	SCCS Opinion
=100	mg/kg	NOAEL	oral	rat	SCCS Opinion
=100	mg/kg/day	NOAEL	oral	rat	SCCS Opinion
=300	mg/kg bw/day	NOAEL	oral	Rat	ECHA
=300	mg/kg/day	NOAEL	-	-	SCCS Opinion
=300	mg/kg bw/day	NOAEL	-	human	SCCS Opinion
=1000	mg/kg/day	NOAEL	oral	rat	SCCS Opinion
=1000	mg/kg bw/day	NOEL	oral	Rat	ECHA
1.000e+4	ppm	Top dose	Dosed feed	Rat	NTP ICE cancer
2.000e+4	ppm	Top dose	Dosed feed	Mouse	NTP ICE cancer
=0.0004	mg/kg	dermal absorption	oral	rat	Regulatory source
=100	mg/kg	dermal absorption	oral	rat	Regulatory source
100	mg/kg/day	developmental toxicity	oral	rat	Regulatory source
300	mg/kg/day	developmental toxicity	-	-	Regulatory source
300	mg/kg/day	developmental toxicity	oral	rat	Regulatory source
100	mg/kg bw/day	genotoxicity	-	rat	Regulatory source

Showing 24 of 24 studies

REACH Registration

Registration status from the ECHA REACH registered substances database.

SOURCE ECHA 1 records

Status	Name	EC Number	Link
Registered	2,2'-[[4-[(2-hydroxyethyl)amino]-3-nitrophenyl]imino]bisethanol	251-410-3	ECHA overview →

Australian Status (AICIS)

Australian industrial chemicals inventory status and applicable conditions.

SOURCE AICIS inventory 1 records

Inventory Status

listed

SCCS Opinions

EU Scientific Committee opinions tied to the matched substance.

SOURCE SCCS Opinion 2 opinions

Opinion	Date	Conclusion	Max Conc.	Concerns
SCCP/1000/06	19 December 2006	safe	2.8%	secondary amine; nitrosamine <50ppb; sensitisation not excluded
SCCP/1035/06	2006-12-19	Based on the information provided, the SCCP is of the opinion that the use of HC Blue n° 2 itself in semi-permanent hair dye formulations at a maximum concentration of 2.8% in the finished cosmetic product does not pose a risk to the health of the consumer, apart from its sensitising potential.	2.8%	Sensitising potential; comprised of both secondary and tertiary amino groups, prone to nitrosation; must not be combined with nitrosating substances; nitrosamine content < 50 ppb.

Cosmetic Safety Profile

Same-CAS cosmetic ingredient record for cross-vertical context.

SOURCE CosIng / Ingredients DB

EU Status

restricted

Max

2.8%

Pharmaceutical Data

Same-CAS pharmaceutical records from drug and bioactivity sources.

SOURCE ChEMBL / DailyMed CHEMBL1561876

ChEMBL Phase

Not listed

Adverse Events

Bioactivity

View full pharmaceutical profile →

Frequently Asked Questions

What is the NOAEL for HC Blue 2?

HC Blue 2 has 24 NOAEL studies in the database. The lowest reported value is 0.0004 mg/kg via oral in rat. Source: SCCS Opinion.

What regulatory lists include HC Blue 2?

HC Blue 2 appears on 3 regulatory/inventory lists including Europe; Personal care; restricted, Europe; Personal care - hair coloring, children; Europe; Personal care. Source: EPA CPDat.

Is HC Blue 2 a carcinogen according to IARC?

HC Blue 2 is classified by IARC as Group 3 — not classifiable as to its carcinogenicity to humans (evaluated 1992). Source: IARC Monographs on the Identification of Carcinogenic Hazards to Humans.

Is HC Blue 2 used in cosmetics?

Yes, HC Blue 2 is also indexed as a cosmetic ingredient under the name HC Blue No. 2. View the full cosmetic safety profile on the ingredient page for detailed safety data, SCCS opinions, and regulatory status.

Where does the safety data for HC Blue 2 come from?

Safety data is sourced from EPA ToxValDB, EPA CPDat, AICIS (Australian Industrial Chemicals Introduction Scheme), EPA DSSTox, IARC Monographs, ECHA REACH, CosIng / Ingredients DB, ChEMBL / DailyMed. All data traces to primary regulatory sources and is updated from official government databases.

Is HC Blue 2 used outside industrial chemicals?

HC Blue 2 also appears in cosmetics, pharmaceutical databases.