NOAEL Data IARC Group 3 Cosmetic Ingredient

4-Nitro-1,2-phenylenediamine

Also known as: 4-Nitro-o-phenylenediamine, 4-13-00-00075, BRN 0608106, EINECS 202-766-3, NCI-C03941 (+8 more)

CAS 99-56-9

4-Nitro-1,2-phenylenediamine (CAS 99-56-9) is a chemical substance; key hazard signal: IARC Group 3. Key regulatory status: 1 regulatory/inventory list, cosmetic ingredient cross-reference; source data from EPA ToxValDB, EPA CPDat, AICIS, SCCS, IARC, ILO ICSC.

View cosmetic safety profile for 4-Nitro-O-Phenylenediamine →

SOURCE NOAEL studies

NOAEL studies

SOURCE Chemical inventory jurisdictions

Regulatory lists

SOURCE EU CLP Annex VI

GHS signal

Not classified

Chemical Identity

CAS, identifiers, formula, and alternate names for the matched substance record.

SOURCE DSSTox identifiers 18 fields

Name

4-Nitro-1,2-phenylenediamine

CAS Number

99-56-9

Molecular Formula

C6H7N3O2

InChI Key

RAUWPNXIALNKQM-UHFFFAOYSA-N

Monoisotopic Mass

153.053826

Synonyms

4-Nitro-o-phenylenediamine4-13-00-00075BRN 0608106EINECS 202-766-3NCI-C039414-Nitro-para-phenylenediamine4-Nitrophenylenediamine4-Nitro-o-phenylene-diamine4-Nitro-1,2-fenylendiaminNitro-o-phenylenediamineUNII-5A9AX7Y0TTp-nitro-o-phenylendiamine

IARC Carcinogen Classification

Carcinogenic hazard classification from IARC monograph evaluations.

SOURCE IARC Monographs on the Identification of Carcinogenic Hazards to Humans 1 records

Group	Meaning	Evaluated	Volume
Group 3	Not classifiable	1987	16, Sup 7

ICSC Chemical Safety

International Chemical Safety Card hazard and exposure summary.

SOURCE WHO/JECFA 1 records

Field	Value
Long-term Effects	Repeated or prolonged contact may cause skin sensitization.

Functional Uses

Industrial and product-use categories associated with this substance.

SOURCE EPA CPDat 2 records

Dye

Regulatory Lists

Inventory, screening, and regulatory list matches from public chemical databases.

SOURCE EPA CPDat 1 records

List	Keyword	Source
Europe; Personal care; restricted	European Commission	CosIng

NOAEL Studies

Toxicology endpoints rendered from public NOAEL study rows.

SOURCE NOAEL studies Showing 26 of 26 studies

Value	Unit	Endpoint	Route	Species	Source
15	mg/kg bw/day	-	oral	dog	Regulatory source
45	mg/kg/day	-	-	rat	Regulatory source
45	mg/kg bw/day	-	-	-	Regulatory source
500	mg/kg	-	oral	rat	Regulatory source
5A9AX7Y0TT	UNII	FDA UNII substance identifier	-	-	openFDA substances
3	IARC group	IARC carcinogenicity classification	-	-	IARC Monographs
3	unitless	IARC group	-	-	NTP ICE cancer
=0.042	mg/kg	NOAEL	oral	rat	SCCS Opinion
=15	mg/kg bw/d	NOAEL	dermal	rat	CIR Safety Assessment
=15	mg/kg bw/day	NOAEL	oral	dog	SCCS Opinion
=15	mg/kg	NOAEL	oral	rat	SCCS Opinion
=45	mg/kg bw/d	NOAEL	dermal	rat	CIR Safety Assessment
=45	mg/kg/day	NOAEL	-	rat	SCCS Opinion
=45	mg/kg bw/day	NOAEL	-	-	SCCS Opinion
=65	%	NOAEL	oral	-	SCCS Opinion
=265	mg/kg/day	NOAEL	-	-	SCCS Opinion
=500	mg/kg	NOAEL	oral	rat	SCCS Opinion
=340	mg/kg bw/day	NOEL	oral	Rat	ToxValDB ECOTOX
=500	mg/kg bw/day	NOEL	oral	Mouse	ToxValDB ECOTOX
750	ppm	Top dose	-	Rat	NTP ICE cancer
7500	ppm	Top dose	-	Mouse	NTP ICE cancer
=0.042	mg/kg	dermal absorption	oral	rat	Regulatory source
=15	mg/kg	dermal absorption	oral	rat	Regulatory source
15	mg/kg bw/day	developmental toxicity	oral	rat	Regulatory source
265	mg/kg/day	developmental toxicity	-	-	Regulatory source
65	%	reproductive toxicity	oral	-	Regulatory source

Showing 26 of 26 studies

Australian Status (AICIS)

Australian industrial chemicals inventory status and applicable conditions.

SOURCE AICIS inventory 1 records

Inventory Status

listed

SCCS Opinions

EU Scientific Committee opinions tied to the matched substance.

SOURCE SCCS Opinion 2 opinions

Opinion	Date	Conclusion	Max Conc.	Concerns
SCCP/1006/06	19 December 2006	not safe	-	genotoxicity; low MoS
SCCP/0980/06	2006-12-19	Based on the information provided, the SCCP is of the opinion that the use of 4-Nitro-o-phenylenediamine itself as an oxidative hair dye substance at a maximum concentration of 0.5% in the finished cosmetic product (after mixing with hydrogen peroxide) does not pose a risk to the health of the consumer, apart from its sensitising potential.	0.5%	Sensitising potential (extremely potent skin sensitiser). Induces mutations in Salmonella in vitro, but not genotoxic in vivo; genotoxicity/mutagenicity studies on finished hair dye formulations still required. IARC Group 3.

Cosmetic Safety Profile

Same-CAS cosmetic ingredient record for cross-vertical context.

SOURCE CosIng / Ingredients DB

EU Status

restricted

Max

Pharmaceutical Data

Same-CAS pharmaceutical records from drug and bioactivity sources.

SOURCE ChEMBL / DailyMed CHEMBL433721

ChEMBL Phase

Not listed

Adverse Events

Bioactivity

View full pharmaceutical profile →

Frequently Asked Questions

What is the NOAEL for 4-Nitro-1,2-phenylenediamine?

4-Nitro-1,2-phenylenediamine has 26 NOAEL studies in the database. The lowest reported value is 0.042 mg/kg via oral in rat. Source: SCCS Opinion.

What regulatory lists include 4-Nitro-1,2-phenylenediamine?

4-Nitro-1,2-phenylenediamine appears on 1 regulatory/inventory list including Europe; Personal care; restricted. Source: EPA CPDat.

Is 4-Nitro-1,2-phenylenediamine a carcinogen according to IARC?

4-Nitro-1,2-phenylenediamine is classified by IARC as Group 3 — not classifiable as to its carcinogenicity to humans (evaluated 1987). Source: IARC Monographs on the Identification of Carcinogenic Hazards to Humans.

Is 4-Nitro-1,2-phenylenediamine used in cosmetics?

Yes, 4-Nitro-1,2-phenylenediamine is also indexed as a cosmetic ingredient under the name 4-Nitro-O-Phenylenediamine. View the full cosmetic safety profile on the ingredient page for detailed safety data, SCCS opinions, and regulatory status.

Where does the safety data for 4-Nitro-1,2-phenylenediamine come from?

Safety data is sourced from EPA ToxValDB, EPA CPDat, AICIS (Australian Industrial Chemicals Introduction Scheme), EPA DSSTox, IARC Monographs, ILO/WHO ICSC, CosIng / Ingredients DB, ChEMBL / DailyMed. All data traces to primary regulatory sources and is updated from official government databases.

Is 4-Nitro-1,2-phenylenediamine used outside industrial chemicals?

4-Nitro-1,2-phenylenediamine also appears in cosmetics, pharmaceutical databases.