Is L-Proline safe in cosmetics?
L-Proline has a safety rating of "EXCELLENT" in our database. EU status: permitted. US status: permitted.
L-Proline
INCI: PROLINE
L-Proline (CAS 147-85-3) is a cosmetic active ingredient functioning as Dominant residue in collagen Gly-X-Y repeat. Major structural component of. NOAEL 50 mg/kg bw/day (cross-referenced against ToxValDB ECHA IUCLID); EU Regulation 1223/2009 status: permitted. Industrial safety data is also available in the chemical safety database. Same-CAS public records also appear in pharmaceutical data.
View industrial chemical safety profile for CAS 147-85-3 →
Auto-generated CPSR Part A draft with source citations
Function
Dominant residue in collagen Gly-X-Y repeat. Major structural component of collagen; among the most common amino acids in cosmetic formulations.
Safety Rating
EXCELLENT
Chemistry & Identifiers
IUPAC Name
(2S)-pyrrolidine-2-carboxylic acid
InChIKey
ONIBWKKTOPOVIA-BYPYZUCNSA-N
Regulatory Status
EU EU Status
permitted
US US Status
permitted
US Notes
Amino acid with GRAS status as direct food additive; CIR-assessed safe class.
For full compliance data across multiple jurisdictions, use the Substance Compliance tool.
Safety Data
Margin of Safety, dermal absorption, and sensitization profile summaries for L-Proline.
Calculate MoS for your specific formulation with the MoS Calculator.
Pre-Calculated Safety Assessment
Pre-calculated NOAEL → SED → MoS audit trail for L-Proline across SCCS product categories, with measured dermal absorption where available.
Dermal Penetration Profile
Skin absorption and penetration characteristics of L-Proline, relevant to systemic exposure and MoS calculations.
Absorption %
3-6%
Penetration Level
surface
Penetration Depth
stratum corneum
Molecular Weight
115.1 Da
Log P
-2.5
Systemic Absorption
minimal
Topical Bioavailability
2-4%
Safety Margin Factor
low
Vehicle Dependent
Yes — absorption varies by formulation
Source: Cosmetic Science
ECHA REACH Registration
European Chemicals Agency REACH dossier and Substances of Very High Concern listing.
Registration Type
Full
Tonnage Band
100 - 1,000 tonnes
Substance Type
SUBSTANCE
SVHC Candidate
No
Additional Ingredient Properties
10 property records from structured ingredient metadata.
acne
{"cas_number": "147-85-3", "anti_acne_mechanism": "anti_inflammatory", "comedogenicity_rating_0_to_5": "0", "acne_type_efficacy": "inflammatory", "sebum_reduction_pct_clinical": "0-5", "prescription_required_usa": "No", "otc_drug_monograph_category": "No", "max_otc_concentration_pct": "No limit", "japan_quasi_drug_anti_acne": "No", "korea_functional_cosmetic_acne": "No", "eu_cosmetic_restriction_a…
anti_aging
{"anti_aging_claim": "collagen-boosting", "mechanism_of_action": "Building block for proteins, NMF component", "target_skin_layer": "epidermis", "collagen_stimulation": "Yes", "elastin_protection": "Mild", "wrinkle_reduction_pct": "8-15", "clinical_study_duration_weeks": "8", "sample_size": "25", "double_blind": "Yes", "placebo_controlled": "Yes", "published_journal": "Int J Cosmet Sci", "improvem…
anti_pollution
{"cas_number": "147-85-3", "ingredient_class": "other", "primary_mechanism": "barrier_reinforcement", "pollution_types_targeted": "PM2_5", "IR_A_protection": "no", "visible_light_protection": "no", "digital_screen_protection_claim": "no", "PAH_neutralization": "no", "ozone_protection": "no", "mitochondrial_protection": "no", "Nrf2_activation": "no", "AhR_pathway_modulation": "no", "NF_kB_inhibitio…
concentration
{"min_concentration_pct": "0.1", "max_concentration_pct": "2.0", "typical_concentration_pct": "1.0", "product_type": "Serum", "function_at_concentration": "Collagen synthesis", "source": "Clinical literature; Supplier TDS"}
dermal_absorption
{"molecular_weight_da": "115.1", "log_p_value": "-2.5", "skin_penetration_level": "surface", "penetration_depth": "stratum corneum", "absorption_pct": "3-6", "systemic_absorption": "minimal", "penetration_enhancer": "no", "occlusion_effect": "no", "vehicle_dependent": "yes", "skin_barrier_effect": "neutral", "bioavailability_topical_pct": "2-4", "safety_margin_factor": "low", "source": "Cosmetic S…
hair_care
{"hair_function": "protein-treatment", "hair_type_suitability": "all", "curl_pattern_suitability": "all", "porosity_suitability": "all", "protein_sensitivity": "protein", "moisture_protein_balance": "yes", "cg_method_approved": "not-a-silicone", "silicone_type": "not-a-sulfate", "sulfate_type": "no", "drying_alcohol": "no", "fatty_alcohol": "no", "heat_protectant": "yes", "color_safe": "strengthen…
humectant
{"cas_number": "147-85-3", "ingredient_class": "humectant/NMF_component/amino_acid", "molecular_weight_Da": "115.13", "chemical_type": "amino_acid", "water_binding_mechanism": "hydrogen_bonding/hygroscopic/osmotic", "water_holding_capacity_g_per_g": "0.4-0.7", "hygroscopicity_at_50_RH": "good", "hygroscopicity_at_80_RH": "excellent", "NMF_component": "yes", "NMF_percentage_natural": "2-4", "TEWL_r…
pediatric_safety
{"safe_for_newborn_0_6m": "yes", "safe_for_infant_6_12m": "yes", "safe_for_toddler_1_3y": "yes", "safe_for_child_3_12y": "yes", "safe_for_teen_12_18y": "yes", "pediatric_max_concentration_pct": "5.0", "adult_max_concentration_pct": "5.0", "pediatric_dermal_absorption_factor": "normal", "diaper_area_safe": "yes", "pediatric_allergy_prevalence": "low", "banned_in_children_products_eu": "no", "banned…
PubChem Annotations
Safety and regulatory annotations from PubChem (NCBI) aggregated sources.
Safety (1)
Carcinogen Classification
No indication of carcinogenicity to humans (not listed by IARC).
Source: Toxin and Toxin Target Database (T3DB)
Regulatory (2)
JECFA Number
1425
Source: Joint FAO/WHO Expert Committee on Food Additives (JECFA)
JECFA Evaluation
L-PROLINE | 2004 | No safety concern at current levels of intake when used as a flavouring agent | Not evaluated using the Procedure for the Safety Evaluation of Flavouring Agents; the substance is a macronutrient and a normal component of protein and, as such, human exposure through food is orders of magnitude higher than the anticipated level of exposure from use as a flavouring agent | TRS 928-
Source: Joint FAO/WHO Expert Committee on Food Additives (JECFA)
Function Categories & List Membership
Cosmetic/Chemical Function Categories
Anti-static agent
Flavouring and nutrient
Fragrance
Softener and conditioner
Regulatory List Membership (2)
EU Regulated in Personal Care
FDA Substances Added to Food (EAFUS)
Expert Verdict
Expert safety assessment and concern-level summary for L-Proline.
Regulatory Flags
fda_food_additive
jecfa_safe
Chemical Function
Anti-static agent
Softener and conditioner
Fragrance
Flavouring and nutrient
Regulatory Lists
Europe
food_additive; Substances Added to Food (formerly EAFUS) (2/2019)
pharmaceutical
active_ingredient; animal_products; Europe; pharmaceutical; residue
Europe; Personal care
Europe; Personal care
Canada; pharmaceutical
Australian Status
AICIS status: listed
Cross-Vertical Safety Context
Same-CAS public records found in pharmaceutical data.
SOURCE
ChEMBL / DrugCentral / FAERS
DB_PHARMA CAS match
Pharmaceutical Data
Same-CAS rows from the pharmaceutical spoke database.
Compound
PROLINE
ChEMBL ID
CHEMBL54922
Max Phase
2
Adverse Event Associations
5
Frequently Asked Questions
Is L-Proline allowed in the EU?
L-Proline EU regulatory status: permitted. This is based on EU Regulation 1223/2009 and its amendments.
What does L-Proline do in cosmetics?
L-Proline functions as: Dominant residue in collagen Gly-X-Y repeat. Major structural component of collagen; among the most common amino acids in cosmetic formulations.. It is classified as a Active Ingredient in our database. CAS number: 147-85-3.
Is L-Proline used outside cosmetics?
L-Proline also appears in pharmaceutical data. The cross-vertical cards on this page render same-CAS public rows from the matched databases.
Compliance Tools
Check L-Proline compliance across multiple jurisdictions:
Ingredient Decoder
Paste a full ingredient list for instant analysis
82 Allergen Checker
Check for EU declarable fragrance allergens
Substance Compliance
Check compliance across multiple jurisdictions
MoS Calculator
Calculate Margin of Safety using SCCS methodology
Heavy Metal Limits
Compare limits across jurisdictions
Related Ingredients
Acetyl Tetrapeptide-5
Excellent
Same category
Anti-edema peptide for periorbital ar...
Activated Charcoal
Excellent
Same category
Adsorbent; skin cleanser; pore-cleans...
Adenosine
Excellent
Same category
Skin-conditioning agent, anti-aging, ...
Allantoin
Excellent
Same category
Skin conditioner, keratolytic, anti-i...
Alpha-Glucan Oligosaccharide
Excellent
Same category
prebiotic, skin-conditioning
Ascophyllum Nodosum Extract
Excellent
Same category
Skin Brightening, Tyrosinase Inhibito...
1-Methylhydantoin-2-imide (Creatinine)
Excellent
Similar safety
Skin conditioning (skin brightening/l...
2,6-Dimethyl-7-octen-2-ol (Dihydromyrcenol)
Excellent
Similar safety
Fragrance (perfuming), masking
2-Ethoxy-2,6,6-Trimethyl-9-Methylenebicyclo[3.3.1]Nonane (Boisiris™)
Excellent
Similar safety
Fragrance (rich woody, orris/iris roo...
2-Hydroxypropylcarbamato Amodimethicone
Excellent
Similar safety
Hair conditioning; modified aminosili...
2-O-Ethyl Ascorbic Acid
Excellent
Similar safety
Softener and conditioner
3 O Ethyl Ascorbic Acid
Excellent
Similar safety
Antioxidant; tyrosinase inhibitor; me...
CI 74160
Good
Chemical family
Colorant
Diphenhydramine HCl
Poor
Chemical family
Skin-conditioning agent (pharmacologi...
Pigment Blue 15
Good
Chemical family
Colorant
Is L-Proline in Your Products?
Scan any product label with Piro — our free ingredient scanner. Point your camera at the ingredients list and get instant safety scores for every ingredient.
Try Piro Free →Analyze Your Full Ingredient List
Paste any INCI list into the Decoder for instant safety analysis, or calculate the Margin of Safety for L-Proline in your formulation.